Journal: PLoS ONE

Article Title: Decellularized scaffold of cryopreserved rat kidney retains its recellularization potential

doi: 10.1371/journal.pone.0173040

Figure Lengend Snippet: Laminin immunohistostaining was carried out using anti laminin sera followed by FITC conjugated secondary IgG representing a) control kidney from group I, b) decellularized structures from freshly isolated kidney, group II and c) decellularized structure from cryostored kidney, group III at 200X. Where ever shown, the white arrows represented renal cells (blue stain) with black arrows representing laminin (brown stain). The cryostorage for 3 month minimally affected the histological appearance and overall presence of ECM component in the decellularized structure, group III in comparison to freshly de-celularized structure, group II.

Article Snippet: Following antigen retrieval the sections were incubated overnight with primary antibodies Collagen1V (Abcam, 1:200), laminin (Boster Bio, 1:200) at 4°C in a humidified chamber.

Techniques: Control, Isolation, Staining, Comparison

Journal: Oncotarget

Article Title: Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway

doi: 10.18632/oncotarget.17144

Figure Lengend Snippet: (A) Representative bands of CollagenI and III and ratio of these proteins to GAPDH. (B) Representative bands of TGF-β1 and MMP9, data from these proteins were normalized to GAPDH. (C) Representative bands of phospho-Smad3 and Smad3 as well as the ratio of these proteins to GAPDH. * P < 0.05, * P < 0.01, ** * P <0.001 vs. sham group, n=6 in each group.

Article Snippet: After blocking with 5% nonfat dry milk, the membranes were then incubated with anti-CollagenI (bs-0578R, Bioss, China), anti-Collagen III (bs-0549R, Bioss, China), anti-TGFβ1 (ab92486, Abcam, USA), anti-α-SMA (ab5694, Abcam, USA), anti-MMP9 (bs-4593R, Bioss, China), anti-Smad3 (#9523, Cell Signaling Technology, USA), p-Smad3 (#9520, Cell Signaling Technology, USA), anti-AIF (ab1998, Abcam, USA), anti-cleaved caspase 3 (#9664, Cell Signaling Technology, USA), anti-caspase 9 (#9508, Cell Signaling Technology, USA), anti-Bcl-2 (ab32124, Abcam, USA), anti-Bax (ab32503, Abcam, USA), anti-GAP43 (ab12274, Abcam, USA), anti-TH (ab112, Abcam, USA), anti-NLRP3 (bs-10021R, Bioss, China), anti-NF-κB p65 (10745-1-AP, Proteintech, USA), anti-p-NF-κB p65 (#3033, Cell Signaling Technology, USA), anti-CD68 (ab76308, Abcam, USA), HIF-1α (R12-2180, Assaybiotech, USA), anti-GAPDH (KC-5G4, Kangchen, China) and anti-β-actin (TA-09, Zhongshan, China) antibodies in 4°C overnight.

Techniques: